MORE MICROSCOPY LAB
Answer in the space provided
- Record the dFOV's for your microscope:
- Under which objective lens or lenses should the coarse adjustment (focus) knob NOT be used? Explain why.
- Make a "dry mount" of the letter "e" and place the slide on the stage so that the "e" is right-side up when you look at it without the microscope. Now, observe under low, medium, and high power. Describe the orientation of the "e" as you view it under the microscope on any power.
- Describe 2 ways that the "e" looks different under high power compared to low power.
- While looking at the "e" under low power, move the slide to the right. In which direction does the "e" appear to move?
- While looking at the "e" under low power, move the slide upwards. In which direction does the "e" appear to move?
- Calculate the actual size (width) of an "e" on low power. Show all your work.
- What would the actual size of the "e" be on high power?
- Prepare a "wet mount" of a piece of coloured paper from a magazine. Examine under low, medium, and high power. Describe how the paper looks different under high power compared to how it looks without a microscope.
- Calculate the actual size (width) or an unwound paper clip on either low, medium, or high power. Show all your work.
- Does the field of view (dFOV) increase or decrease in size as you move from low to medium to high power?
- Does the light intensity increase or decrease as you move from low to medium to high power?
- "Resolution" is the ability of a microscope to show close objects (like dots) as distinct or separate images. Under which objective is the resolution the greatest?
- How many cells can fit across the high power field of view of your microscope if the actual size (width) of a cell is 0.15 mm? Show all your work.